No More Sales-DynaMarker® Protein MultiColor Stable II, Large

Product#: Inactivated-DM660L
$99,999.00
Availability:
Ships in 1-2 Weeks

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DynaMarker® Protein MultiColor Stable II

Cat. No. DM660 L
Size 5x1.2 ml

Storage 4 °C
Stability 12 months at 4 °C
Storage Buffer Tris-HCl (pH6.8), EDTA, Glycerol, SDS

 

Protein MultiColor Stable Ⅱ
       *This is a renewal product of Protein MultiColor Ladder Marker, Stable.

Renewal Points !

*The product name changes from "Stable" to "StableⅡ".
*Aprotinin (Green) of 8.5kDa is newly added.
*The loading amount per one lane changes from 5ul to 10ul. Number of loads do not change, 120 times.

Description
Pre-stained protein ladder for broad range of proteins (8 to 230 kDa), stable in refrigerator ( at 4℃ ) for 1 year !
 
The DynaMarker® Protein MultiColor Stable II is a pre-stained protein molecular weight marker. The marker has a remarkable feature that it is possible to store at 4 °C. The feature allow us to start electrophoresis with the marker, because it is always in a liquid state while stored at 4 °C. The DynaMarker® Protein MultiColor Stable II consists of nine prestained proteins. Each of them are stained red, blue, purple, green or orange, ranging in apparent molecular weight from approximately 8 kDa to 230 kDa. The DynaMarker® Protein MultiColor Stable II is suitable for visualizing proteins during electrophoresis without staining and for monitoring electrophoretic transfer onto membranes. The protein concentrations are optimized to give uniform band intensities. The marker is supplied in gel loading buffer for direct loading onto SDS-PAGE without heating or adding reducing agents.
DM660_fig1Description.png  DM660_fig2.png
 Electrophoresis profile of DynaMarker Protein MultiColor Stable II (10 μl) on 6% polyacrylamide (5% C) Gel / AllView PAGE Buffer (Code#DS520) as running buffer.

Feature
  • Multicolored pre-stained natural proteins
  • Stable for up to 1 year at 4℃ 
  • Ready-to-load (10μL / lane)
  • Sharp bands
  • M.W. range from approx. 8 to 230 kDa
Applications
  • Real-time monitoring of protein mobility on SDS-PAGE gel.
  • Can check transfer efficiency on membranes without staining.
Protocol
  1. Take the marker out of refrigerator.
  2. Load 10 μl for mini-gels or more for large size gels.
  3. Load your samples.
  4. Start electrophoresis.
Note There is no need to heat or add reducing agents

Contents
Apparent molecular weight (kDa) *

Protein

Tris-Glycine-SDS buffer (Laemmli running buffer)

AllView PAGE Buffer (Code#DS520)

Myosin

249

250

β-Galactosidase

137

141

Phosphorylase-b

96

100

BSA

73

71

Ovalbumin

46

45

Carbonic anhydrase

31

30

Soybean trypsin inhibitor

26

25

Lysozyme

18

17

Aprotinin

6.3

8.4


Note
As covalently bound dye affects protein mobility, each batch of prestained protein marker is calibrated against
unstained standards. A prestained protein marker should be used for approximate molecular weight determination. For
precise molecular weight determination use an unstained molecular weight marker.

* : The apparent molecular weight values are lot specific and depends on the electrophoresis running buffer.

Comparison
 

Supplier

BioDynamics Laboratory (BDL)

Company A

Company B

Company C

Company D

Number of loads

120

50

50

50

50

Storage Temp.

4

-20

-20

4

4

Shelf Life

1 year

3 months

1 year

4 months

3 months


Related product
 

Code

Product Name

Description

DS520



 

All View PAGEBuffer 



 

AllView PAGE Buffer is a new type of running buffer for SDS-PAGE electrophoresis. This buffer is possible to separate proteins with wide range of molecular weights in the basic Laemmli gel (Tris-HCl) similar to using “gradient gel”.
 

 
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